File "bacteria" contains 10870 sequences:
1. search NCBI using "bacteria[ORGN] AND 16s[TITL]" got 81343 sequence.
2. Leftprimer: "AGRRTTTGATYHTGGYTCAG"
RightPrimer: "AAGTCGTAACAAGGTAVC"
Mismatch: 3 ; Minlen:1350 ; Maxlen: 1550
3. Sequences containing primers: PTNDB(3125);
Sequences are homologs: HOMODB(8594)
Overall rDNA sequence: rRNADB (11719) without redundant moving yet.
10870 sequences left with duplicated sequences removed.
File "fungi" contains 1168 sequences:
1. search NCBI using "18s[TITL] AND fungi[ORGN]" got 15933 sequence.
2. Leftprimer: "TTAGCATGGAATAATRRAATAGGA"
RightPrimer: "TGGGATAGRGCATTGCAAT"
Mismatch: 0 ; Minlen:0 ; Maxlen: 10000
3. 1592 sequences selected out, 217 detergent seqence.
1168 sequences left with duplicated sequences removed.
File "new fungi" contains 1245 sequences:
1. Fungal Primers:
Fow: "TTAGCATGGAATAATRRAATAGGA"
Rev: "ATTGCAATGCYCTATCCCCA"
77 sequences
2. Add these 77 sequences to the fungi 1168 file, get 1245 sequences.